Protein_Domain

Part:BBa_K2003000:Design

Designed by: Jakob Nyberg   Group: iGEM16_Uppsala   (2016-09-01)


CPF1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1454
    Illegal BglII site found at 1646
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CPF1 from Francisella Novicida. A his-tag was added for easier IMAC purification.


Source

Synthezised by IDT. Sequence codon optimized for E.coli.

References

Fonfara, I., Richter, H., Bratovič, M., Le Rhun, A., & Charpentier, E. (2016). The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA. Nature, 532(7600), 517-521. ISO 690